ID: 410
Titel: Variabilitet av biomarkörer i blod och urin
Provsamlingsansvarig: Gerd Sällsten
Start insamling: 2012-01-01
Slut insamling: 2013-12-31
Beskrivning: Blood and spot urine samples were collected from 60 healthy non-smoking study participants 18-65 years of age (29 men and 31 women; no diabetes, hypertension, or kidney disease). The spot urine samples were collected during two 24h periods about one week apart, and the study participants were instructed to urinate at six fixed times (first morning void, 9:30, 12:00, 14:30, 17:30, and 22:00). The samples were transferred to Minisorb tubes (NUNC) and kept at 4°C until analysis of proteins (Albumin and Alpha-1-microglobulin), creatinine and density within 3 days of collection.  Blood samples were collected twice, one in each week. Aliquots of urine and blood (blood, plasma and erythrocytes) are stored frozen (-80°C). The biobank (more than 20 000 aliquots) is now open for researchers interested in examining normal variability of their favorite biomarker(s). 
The biobank is especially suitable for analysis of diurnal variation in urine biomarkers (12 samples per subject), but could also be used for analyses of variability in biomarker concentrations in blood, plasma or red cells. Variability in certain biomarkers using a previous, smaller biobank have been published for cadmium and proteins (Akerstrom M et al., JEESE 2014), and 8-oxodG (Barregard L et al., ARS 2013).


PROVTYP;PROVGIVARE
urin, 09:30;60
urin, 09:30;60
urin, 12:00;60
urin, 12:00;60
urin, 14:30;60
urin, 14:30;60
urin, 17:30;60
urin, 17:30;60
urin, 22:00;60
urin, 22:00;60
urin, första morgonurin/över natten;60
urin, första morgonurin/över natten;60
helblod, tillfälle 1/Vacuette Li-heparin;60
helblod, tillfälle 2/Vacuette Li-heparin;60
plasma, tillfälle 1/BD368886;60
plasma, tillfälle 2/BD368886;60
helblod, ery tillfälle 1/BD368886;60
helblod, ery tillfälle 2/BD368886;60
plasma, tillfälle 1/BD367704;60
plasma, tillfälle 2/BD367704;60